ATP + [[protein|PtsH|HPr]] = ADP + P-Ser-[[protein|PtsH|HPr]] (according to Swiss-Prot)

[HPr protein]-L-serine + ATP --> [HPr protein]-O-phospho-L-serine + ADP + H⁺ (according to UniProt)

[HPr protein]-O-phospho-L-serine + H⁺ + phosphate --> [HPr protein]-L-serine + diphosphate (according to UniProt)

for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP642, available in [SW|Jörg Stülke]'s lab

for expression/ purification of mutant HprK-G158A from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP650, available in [SW|Jörg Stülke]'s lab

for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP205, available in [SW|Jörg Stülke]'s lab

for expression, purification of the N-terminal in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP218, available in [SW|Jörg Stülke]'s lab

for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP642, available in [SW|Jörg Stülke]'s lab

for expression/ purification of mutant HprK-G158A from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP650, available in [SW|Jörg Stülke]'s lab

for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP205, available in [SW|Jörg Stülke]'s lab [pubmed|12055300]

for expression, purification of the N-terminal domain in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP218, available in [SW|Jörg Stülke]'s lab